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本試劑盒適用于乳品等樣品中呋喃唑酮的定性或定量測定<br> </article> </div>   <div class="e_box d_PriceBox p_PriceBox background_skuBg" data-ename="價格信息區"> <div class="e_box d_SalePriceBox p_SalePriceBox" data-ename="批發價格信息區"> <div class="e_title d_nameG p_nameG" data-ename="批量價引導標題"> <div class="font">零售價</div> </div> <div class="e_title d_SalePrice p_SalePrice price" data-ename="批發價"> <div class="font">0.0</div> </div> <div class="e_title d_unit p_unit" data-ename="計量單位"> <div class="font">元</div> </div> </div> <div class="e_box d_MarketPriceBox p_MarketPriceBox" data-ename="市場價格信息區"> <div class="e_title d_nameH p_nameH" data-ename="市場價引導標題"> <div class="font">市場價</div> </div> <div class="e_title d_MarketPrice p_MarketPrice" data-ename="市場價"> <div class="font"></i>0.0</div> </div> <div class="e_title d_unitA p_unitA" data-ename="計量單位"> <div class="font">元</div> </div> </div>  <div class="e_box p_ViewBox" data-infoid="35" data-ename="瀏覽數量信息區"> <div class="e_title p_AssistNameB" data-ename="瀏覽數量引導標題"> <div class="font"> <i class="iconfont"></i>瀏覽量:</div> </div> <div class="e_title p_view" data-ename="瀏覽數量"> <div class="font"> <i class="iconfont"></i> 1010</div> </div> </div> </div>  <div class="e_box d_NumBox p_NumBox" data-ename="編號區"> <div class="e_title d_nameC p_nameC" data-ename="編號引導標題"> <div class="font">產品編號</div> </div> <div class="e_title d_num p_num" data-ename="編號"> <div class="font"></div> </div> </div>   <div class="e_box d_SortName p_SortName" data-ename="分類信息區"> <div class="e_title d_nameD p_nameD" data-ename="分類引導標題"> <div class="font">所屬分類</div> </div> <a href="/product/食品安全檢測產品（酶聯免疫）-5" class="e_link d_linkA p_linkA" target="_blank" style="float: right"> <div class="e_title p_sort" data-ename="分類名稱"> <div class="font"> <i class="iconfont"></i>食品安全檢測產品（酶聯免疫）</div> </div> </a> </div>   <div class="e_box p_products"></div>   <div class='e_box d_NumberBox p_NumberBox_'> <div class='e_title d_nameM p_nameM_'> <div class="font"> 數量 </div> </div> <div class="e_btn d_minus p_minus border_default background_form"> <div class="font">-</div> </div> <input id="buyNum" name="name" type="text" class='e_input d_input p_input border_default' value=""> <div class="e_btn d_plus p_plus border_default background_form"> <div class="font">+</div> </div> <div class="e_title d_nameO p_nameO" data-ename="庫存引導標題"> <div class="font">庫存:</div> </div> <div class="e_title d_numA p_numA js_stockNum" data-ename="庫存數量"> <div class="font"></div> </div> </div> </div>  <div class="e_box p_interaction" data-ename="互動按鈕區">  <input type="hidden" class="js_compareFloatData" id="35" name="呋喃唑酮說明書" templateId="1" image="repository/image/OczosS73S5mcb4kaV33WyA.jpg_135x135f.jpg"> <div class="Prodownload hide"> </div>  <div class="e_box p_shopCartBox dom_mo" data-ename="購物車容器"> <div class="e_box p_shopCart h1" data-ename="購物車"> <div class="font"> <i class="iconfont"></i> </div> </div>  <div class="e_box p_shopCartNum background_shopCart color_horizon h6" data-ename="購物車數量"> <div class="font">1</div> </div>  </div>  </div>  </div> <div class="e_box d_DescriptionBoxA p_DescriptionBoxA " data-ename="詳情信息區">  <div class="e_box d_TabTitleBox p_TabTitleBox" data-ename="頁簽區">  <div class='e_RelationBtn d_DecTab decTab active' id="contentPage"> <div class="d_DecTabA p_DecTabA_1"> <div class="font">產品描述</div> </div> </div>  </div>   <div class="e_box d_DescriptionBigbox p_DescriptionBigbox borderT_default" data-ename="頁簽內容區">  <div id="contentDiv" class='e_box d_DescribeContent p_DescribeContent' data-ename="描述內容區">  <div class="e_box d_BaseInfoBox p_BaseInfoBox background_form js_attrOne"> </div> <div class='e_HtmlEditor d_description'> <div class='reset_style js-reset_style js-adapMobile '><h3 style="text-align: center;"> 呋喃唑酮一步法ELISA試劑盒</h3> <h3 style="text-align: center;"> 使用說明書</h3> <div> <strong>1適用范圍</strong></div> <div> 本試劑盒適用于乳品等樣品中呋喃唑酮的定性或定量測定</div> <div> 檢測數量：96孔（含標準品）</div> <div> 檢測時間：40分鐘</div> <div> <strong>2檢測原理</strong></div> <div> 樣品中游離的呋喃唑酮代謝物與酶標記物競爭結合微孔中固相包被的呋喃唑酮代謝物特異性抗體，經過孵育后進行清洗，所有未與抗體結合的藥物在清洗步驟中被清除，與抗體結合的酶標記物由顯色劑顯示出來，因為酶可以使無色的顯色劑轉變成藍色，終止液的加入可以終止顯色，藍色轉變成黃色，吸收值可以在450nm處進行測定。顏色變化的程度反映樣品中代謝物的含量</div> <div> <strong>3提供的材料</strong></div> <div> 3.1包被有抗體的微孔板：1塊（12條×8孔，可拆分）</div> <div> 3.2酶標物工作液：1瓶（7mL）</div> <div> 3.3樣品稀釋液10×：1瓶（20mL）</div> <div> 3.4清洗液20×：1瓶（25mL）</div> <div> 3.5顯色劑：1瓶（13mL）</div> <div> 3.6終止液：1瓶（13mL）</div> <div> 3.7呋喃唑酮代謝物標準品： 6×1mL/瓶（0，200ng/ml，500 ng/ml，2000 ng/ml，4000 ng/ml，6500 ng/ml）</div> <div> 3.8說明書</div> <div> 3.9質檢報告</div> <div> <strong>4需要而未提供的材料</strong></div> <div> 4.1不同規格的微量移液器</div> <div> 4.250~300μL多通道微量移液器</div> <div> 4.3酶標儀（配有450nm濾光片和630nm濾光片）</div> <div> 4.4去離子水</div> <div> 4.5計時器</div> <div> <strong>5工作液準備與試劑配制</strong></div> <div> 6.1標準品：將各標準品從泡沫墊中取出，回溫，備用</div> <div> 6.2酶標物工作液：將酶標物工作液從泡沫墊中取出，回溫，備用</div> <div> 6.3樣品稀釋工作液：將樣品稀釋液10×（見4.3）用去離子水按1:10（1+9）稀釋，備用</div> <div> 6.4清洗液：將清洗液20×（見4.4）用去離子水按1:20（1+19）稀釋，備用</div> <div> 6.5顯色劑：已備用，避免光線直照</div> <div> 6.6終止液：已備用</div> <div> <strong>6注意事項</strong></div> <div> 7.1使用試劑盒前請仔細閱讀說明書</div> <div> 7.2不同試劑盒中的試劑不得混用</div> <div> 7.3終止液、底物液有刺激性，請勿接觸皮膚</div> <div> 7.4使用前，請將試劑盒中所有試劑置于22~25℃條件下回溫1小時以上，一定保證回溫充分，否則影響檢測的精確度和準確度</div> <div> 7.5多余的微孔重新密封后立即于2~8℃干燥保存</div> <div> 7.6由于實驗結果的準確度很大程度上與操作有關，在實驗操作過程中請不要改變分析步驟和中斷任何程序</div> <div> 7.7請使用精確微量移液器</div> <div> 7.8為避免交叉污染，每個標準品和樣品均應使用不同的吸頭移取液體</div> <div> 7.9加樣時請勿讓吸頭接觸微孔中的溶液或內表面</div> <div> 7.10試劑盒使用過程中必需使用蓋板膜。</div> <div> <strong>7操作步驟</strong></div> <div> 8.1 確認樣品已完成前處理或已備用，所用產品試劑已備用并回溫充分</div> <div> 8.2 預先進行編號，標記標準品和樣品的位置，建議進行雙孔平行實驗</div> <div> 8.3 從微孔板上取下所需數量的微孔（將多余的重新密封保存）</div> <div> 8.4 在各標準孔中加入50μL的標準品溶液，在各樣品孔中加入50μL樣品溶液</div> <div> 8.5 立即在所有孔中加入50μL的酶標物工作液（見6.2）（強烈推薦多通道移液器以及加樣槽配合使用）</div> <div> 8.6 輕輕晃動反應板幾秒鐘，用蓋板膜封板，37℃條件下孵育30分鐘</div> <div> 8.7 倒掉微孔中的液體，在所有微孔中加入250μL清洗液，輕輕敲動反應板，然后倒掉微孔內液體</div> <div> 8.8 重復8.7步驟3遍，總共洗板4次</div> <div> 8.9 將微孔板倒扣在吸水紙上拍干，徹底清除掉微孔中的殘留液和氣泡（拍干后立即進行下一步驟，切勿讓微孔干燥）</div> <div> 8.10清洗程序完成后，立即向每個微孔中加入100μL顯色劑（建議使用多通道移液器），晃動反應板使之混勻</div> <div> 8.11在22~25℃條件下顯色10分鐘</div> <div> 8.12向每個微孔中加入50μL終止液（建議使用多通道移液器）</div> <div> 8.13在450nm和630nm下檢測吸光度，結果在10分鐘內讀取有效,讀取的數據輸入半對數曲線進行含量分析</div> <div> 8.14實驗結束后應將未用完的試劑裝回試劑盒封好，于2~8℃保存</div> <div> <strong>8結果計算</strong></div> <div> 9.1計算每個標準品和樣品的平均吸光度</div> <div> 所有標準品和樣品的平均吸光度除以零標準的平均吸光度，再乘以100，即得以%表示的結合率：</div> <div> 標準（或樣品）的吸光度B</div> <div> 零標準的吸光度B0</div> <div> 9.2將每個標準的B/ B0值輸入半對數系統中，對應于不同標準品的濃度可以獲得一個標準曲線</div> <div> 9.3用樣品的半對數曲線中就可以找到對應的濃度</div> <div> 9.4在標準曲線中得到的濃度結果還要最后乘上相應的稀釋倍數，獲得樣品稀釋前實際的呋喃唑酮代謝物含量，用ppb（μg /kg或μg /L）表示</div> <div> <strong>9貯存及有效期</strong></div> <div> 14.1呋喃唑酮一步法ELISA試劑盒在2-8℃條件貯存</div> <div> 14.2試劑盒有效期：12個月（請勿使用過期產品）</div> </div> </div>    <div class="e_box p_QRCode " data-ename="打開手機查看"> <div class="e_title p_SaveText " data-ename="二維碼引導信息"> <div class="font"> 掃二維碼用手機看 </div> </div> <div class="e_image js_qrcode" data-ename="二維碼"></div> </div> </div>  <div id="paramDiv" class='e_box hide d_ProParametersBox p_ProParametersBox border_default' data-ename="產品參數容器">  <div 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