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data-ename="產品詳情容器"> <div class="c_portalResProduct_detail-15583500306498182-imgjs" data-innerdismoveable></div> <div class="e_box p_assist" data-ename="互動信息組">  <div class="e_box p_ViewBox" data-infoid="27" data-ename="瀏覽數量信息區"> <div class="e_title p_AssistNameB" data-ename="瀏覽數量引導標題"> <div class="font"> <i class="iconfont"></i>瀏覽量:</div> </div> <div class="e_title p_view" data-ename="瀏覽數量"> <div class="font"> <i class="iconfont"></i> 1012</div> </div> </div> </div> </div>  <div class="e_box p_ProInfo" data-ename="主要信息區"> <div class="e_box p_content borderB_dividers" data-ename="重要信息區">  <div class="e_box d_ProName p_ProName" data-ename="標題區"> <h1 class="e_title d_Title p_Title h2" data-ename="名稱"> <div class="font"> <i class="iconfont"></i>四環素說明書</div> </h1> </div>   <div class="e_box d_ProSummary p_ProSummary" data-ename="概要描述區"> <article class="e_article d_summary p_summary" data-ename="概要描述"> 檢驗原理 :<br> 樣品中游離的四環素與微孔中固相包被的四環素抗原特異性競爭結合酶標抗體，:<br> 包裝規格 ::<br> 96人份/盒:<br> </article> </div>   <div class="e_box d_PriceBox p_PriceBox background_skuBg" data-ename="價格信息區"> <div class="e_box d_SalePriceBox p_SalePriceBox" data-ename="批發價格信息區"> <div class="e_title d_nameG p_nameG" data-ename="批量價引導標題"> <div class="font">零售價</div> </div> <div class="e_title d_SalePrice p_SalePrice price" data-ename="批發價"> <div class="font">0.0</div> </div> <div class="e_title d_unit p_unit" data-ename="計量單位"> <div class="font">元</div> </div> </div> <div class="e_box d_MarketPriceBox p_MarketPriceBox" data-ename="市場價格信息區"> <div class="e_title d_nameH p_nameH" data-ename="市場價引導標題"> <div class="font">市場價</div> </div> <div class="e_title d_MarketPrice p_MarketPrice" data-ename="市場價"> <div class="font"></i>0.0</div> </div> <div class="e_title d_unitA p_unitA" data-ename="計量單位"> <div class="font">元</div> </div> </div>  <div class="e_box p_ViewBox" data-infoid="27" data-ename="瀏覽數量信息區"> <div class="e_title p_AssistNameB" data-ename="瀏覽數量引導標題"> <div class="font"> <i class="iconfont"></i>瀏覽量:</div> </div> <div class="e_title p_view" data-ename="瀏覽數量"> <div class="font"> <i class="iconfont"></i> 1012</div> </div> </div> </div>  <div class="e_box d_NumBox p_NumBox" data-ename="編號區"> <div class="e_title d_nameC p_nameC" data-ename="編號引導標題"> <div class="font">產品編號</div> </div> <div class="e_title d_num p_num" data-ename="編號"> <div class="font"></div> </div> </div>   <div class="e_box d_SortName p_SortName" data-ename="分類信息區"> <div class="e_title d_nameD p_nameD" data-ename="分類引導標題"> <div class="font">所屬分類</div> </div> <a href="/product/食品安全檢測產品（酶聯免疫）-5" class="e_link d_linkA p_linkA" target="_blank" style="float: right"> <div class="e_title p_sort" data-ename="分類名稱"> <div class="font"> <i class="iconfont"></i>食品安全檢測產品（酶聯免疫）</div> </div> </a> </div>   <div class="e_box p_products"></div>   <div class='e_box d_NumberBox p_NumberBox_'> <div class='e_title d_nameM p_nameM_'> <div class="font"> 數量 </div> </div> <div class="e_btn d_minus p_minus border_default background_form"> <div class="font">-</div> </div> <input id="buyNum" name="name" type="text" class='e_input d_input p_input border_default' value=""> <div class="e_btn d_plus p_plus border_default background_form"> <div class="font">+</div> </div> <div class="e_title d_nameO p_nameO" data-ename="庫存引導標題"> <div class="font">庫存:</div> </div> <div class="e_title d_numA p_numA js_stockNum" data-ename="庫存數量"> <div class="font"></div> </div> </div> </div>  <div class="e_box p_interaction" data-ename="互動按鈕區">  <input type="hidden" class="js_compareFloatData" id="27" name="四環素說明書" templateId="1" image="repository/image/o04zWV03SmKVvAWK4Z7JxQ.jpg_135x135f.jpg"> <div class="Prodownload hide"> </div>  <div class="e_box p_shopCartBox dom_mo" data-ename="購物車容器"> <div class="e_box p_shopCart h1" data-ename="購物車"> <div class="font"> <i class="iconfont"></i> </div> </div>  <div class="e_box p_shopCartNum background_shopCart color_horizon h6" data-ename="購物車數量"> <div class="font">1</div> </div>  </div>  </div>  </div> <div class="e_box d_DescriptionBoxA p_DescriptionBoxA " data-ename="詳情信息區">  <div class="e_box d_TabTitleBox p_TabTitleBox" data-ename="頁簽區">  <div class='e_RelationBtn d_DecTab decTab active' id="contentPage"> <div class="d_DecTabA p_DecTabA_1"> <div class="font">產品描述</div> </div> </div>  </div>   <div class="e_box d_DescriptionBigbox p_DescriptionBigbox borderT_default" data-ename="頁簽內容區">  <div id="contentDiv" class='e_box d_DescribeContent p_DescribeContent' data-ename="描述內容區">  <div class="e_box d_BaseInfoBox p_BaseInfoBox background_form js_attrOne"> </div> <div class='e_HtmlEditor d_description'> <div class='reset_style js-reset_style js-adapMobile '><h3 style="text-align: center;"> 四環素檢測試劑盒（酶聯免疫法）說明書</h3> <div> 【產品名稱】</div> <div> 四環素檢測試劑盒（酶聯免疫法）</div> <div> 【包裝規格】</div> <div> 96人份/盒</div> <div> 【預期用途】</div> <div> 本試劑盒適用于乳品中四環素的定性或定量測定。</div> <div> 【檢驗原理】</div> <div> 樣品中游離的四環素與微孔中固相包被的四環素抗原特異性競爭結合酶標抗體，經過孵育后清洗，所有未與抗體結合的藥物在清洗步驟中被清除，與抗體結合的酶標記物由顯色劑顯示出來，因為酶可以使無色的顯色劑轉變為藍色，終止液的加入可以終止顯色，藍色轉變成黃色，吸收值可以在450nm處進行測定。顏色變化的程度反映樣品中四環素的含量。</div> <div> 【主要組成成分】</div> <div> 1.酶標板：1塊（96孔）</div> <div> 2.標準品：6瓶，0.5mL/瓶。TE濃度分別為0ng/mL，1ng/mL，2.5ng/mL，5ng/mL，10ng/mL，25ng/mL。</div> <div> 3.HRP標記的抗TE抗體：1×6mL/瓶。</div> <div> 4.樣品稀釋液10×25mL/瓶。</div> <div> 5.底物液：1×10mL/瓶。</div> <div> 6.20×洗液：1×30mL/瓶。</div> <div> 7.終止液：1×6mL/瓶。</div> <div> 8.說明書：1份。</div> <div> 9.自封袋：1個。</div> <div> 10.封板膜：2張。</div> <div> 【儲存條件及有效期】</div> <div> 本試劑盒請于2～8℃保存。</div> <div> 【適用儀器】</div> <div> 適用于8×12微孔板的酶標儀和洗板機。</div> <div> 【工作液準備與試劑配制】</div> <div> 1.標準品，酶標物：將各標準品，酶標物從泡沫墊中取出，回溫，備用。</div> <div> 2.清洗液：將清洗液20×用去離子水按1:20稀釋，備用。</div> <div> 3.樣品稀釋工作液：將樣品稀釋液10×用去離子水按1:10稀釋，備用。</div> <div> 4.顯色劑：已備用，避免光線直照。</div> <div> 5.終止液：已備用。</div> <div> 【注意事項】</div> <div> 1.使用試劑盒前請仔細閱讀說明書。</div> <div> 2.不同試劑盒中的試劑不得混用。</div> <div> 3.終止液、顯色劑有刺激性，請勿接觸皮膚。</div> <div> 4.使用前，請將試劑盒中所有試劑置于22~25℃條件下回溫1小時以上，一定保證回溫充分，否則影響檢測的精確度和準確度。</div> <div> 5.多余的微孔重新密封后立即于2~8℃干燥保存。</div> <div> 6.由于實驗結果的準確度很大程度上與操作有關，在實驗室操作過程中請不要改變分析步驟和中斷任何程序。</div> <div> 7.請使用精確微量移液器。</div> <div> 8.為避免交叉污染，每個標準品和樣品均應使用不同的吸頭移取液體。</div> <div> 9.加樣時請勿讓吸頭接觸微孔中的溶液或內表面。</div> <div> 【操作步驟】</div> <div> 1.確認樣品已完成前處理或已備用，所用產品試劑已備用并回溫充分。</div> <div> 2.預先進行編號，標記標準品和樣品的位置，建議進行雙孔平行實驗。</div> <div> 3.從微孔板上取下所需數量的微孔（將多余的重新密封保存）。</div> <div> 4.在各標準孔中加入50uL的標準品溶液，在各樣品孔中加入50uL樣品溶液。</div> <div> 5.立即在所有孔中加入50uL的酶標物溶液。</div> <div> 6.輕輕晃動反應板幾秒鐘，37℃條件下孵育30分鐘。</div> <div> 7.倒掉微孔中的液體，在所有微孔中加入250uL清洗液，輕輕敲動反應板，然后倒掉微孔內液體。</div> <div> 8.重復步驟7，總共洗板5次。</div> <div> 9.將微孔板倒扣在吸水紙上拍干，徹底清除掉微孔中殘留液和氣泡（拍干后立即進行下一步驟，切勿讓微孔干燥）。</div> <div> 10.清洗程序完成后，立即向每個微孔中加入100uL顯色劑，晃動反應板使之混勻。</div> <div> 11.在37℃條件下顯色10分鐘。</div> <div> 12.向每個微孔中加入50uL終止液。</div> <div> 13.在450nm下檢測吸光度，結果在10分鐘內讀取有效，讀取的數據輸入半對數曲線進行含量分析。</div> <div> 14.實驗結束后應將未用完的試劑裝回試劑盒封好，于2~8℃保存。</div> <div> 【結果計算】</div> <div> 1.計算每個標準品和樣品的平均吸光度。</div> <div> 所有標準品和樣品的平均吸光度除以零標準的平均吸光度，再乘以100，即得以%表示的結合率：</div> <div> 2.將每個標準品的B/B0值對應于不同標準品的濃度可以獲得一個標準曲線。</div> <div> 3.用樣品的B/B0值在標準曲線中就可以找到對應的四環素的濃度。</div> <div> 4.在標準曲線中得到的濃度結果還要最后乘上相應的稀釋倍數，獲得樣品稀釋前實際的四環素含量，用ppb（ug/kg或ng/mL）表示。</div> <div> 【樣品前處理步驟】</div> <div> 純奶、花色奶</div> <div> 1.稀釋倍數：15倍。</div> <div> 2.取100uL樣品，加入700uL樣品稀釋工作液，震蕩混勻。</div> <div> 3.移取50uL用于檢測。</div> <div> 【產品性能指標】</div> <div> 檢測范圍：0ng/mL~25ng/mL</div> <div> 【貯存及有效期】</div> <div> 1.本試劑盒在2~8℃條件貯存。</div> <div> 2.有效期：12個月。</div> <div> 【基本信息】 </div> <div> 注冊人/生產企業名稱：杭州飛悅?？瞪锟萍加邢薰?/div> <div> 住所：浙江省杭州市余杭區</div> <div> 聯系方式：0571-88753620</div> <div> 售后服務單位名稱：杭州飛悅?？瞪锟萍加邢薰?/div> <div> 聯系方式：0571-89196609</div> <div> 生產地址：浙江省杭州市余杭區</div> <div> 【說明書核準及修改日期】</div> </div> </div>    <div class="e_box p_QRCode " data-ename="打開手機查看"> <div class="e_title p_SaveText " data-ename="二維碼引導信息"> <div class="font"> 掃二維碼用手機看 </div> </div> <div class="e_image js_qrcode" data-ename="二維碼"></div> </div> </div>  <div id="paramDiv" class='e_box hide 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